After a lot of reasoning, thinking, formalizing, making errors in derivatives,
graduating (Pietro), we are currently releasing a new chapter in our replication saga.
On the interaction of replication and transcriptional regulation
Take home messages:
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Replication introduces position- and division time-dependent patterns in the sensitivity of a gene to the activity of its regulator.
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The interaction of copy number variations introduced by changes in division time with
a regulator’s activity can be constructive or destructive. In the first, correlations with copy number and the activity of the regulator
are detectable in the output, in the second, a partial decoupling from copy numbers or regulator’s activity introduces an additional
gauge for gene expression modulation
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Considering replication could be important for (i) gene regulatory network reconstruction, (ii)
promoter and regulator kinetic parameter estimation and (iii) dispersion factor estimation across different conditions in RNA-seq data analysis.
Check the news for new chapters!
Now we will explore how replication may affect the degree and nature of
genome structural variations.
Still working on it; for the moment, mostly working.
Most of the work done in the laboratory of Emanuele Biondi at the Plant-Bacteria interaction Team in Gif-sur-Yvette (Paris, France).
Our modest contribution makes us proud that the collaboration is ongoing after ~15 years!
Check it out here
Check this beauty, it’s out! A renewed working group to explore how operons and higher order gene clusters assemble in time. We use theoretical considerations and results from Metabolic Control Analysis to highlight that once we consider copy number variations introduced by chromosome replication into the equations of a metabolic systems..metabolite pool are perturbed. A way to minimize them? Operons!
Failing is normal. Do it with elegance.
Sometimes I have found myself talking to people who say they only succeed at everything they do. This shocked me, so I probed further. It turns out that this was because they only ever try anything that they know they are good at, knowing that they will be successful, terrified of failure or being imperfect. This seems like a huge shame and a tremendous waste, missing out on all of the surprising twists and turns that life can take us, opting to turn down opportunities due to this aura of insecurity. I am immensely proud of my failures, and I don’t think that I could have achieved a fraction of what I have accomplished if it wasn’t for all of the things that went “wrong”. How can we expect to learn and grow if we make sure that we only succeed 100% of the time? Our achievements are surely that much more impressive if we consider what it has taken to get there.
Hannah Dalgleish source